Case reports of two children with neurofibromatosis type 1 / 中国热带医学

@#Abstract: Objective　To report the clinical characteristics and genetic test results of two children with neurofibromatosis type 1 (NF1), and to provide reference for the comprehensive diagnosis-treatment and follow-up plans of NF1 patients based on the existing diagnosis and treatment progress of NF1. Methods　Two children with NF1 admitted to the Department of Children's Medicine, Haikou people's Hospital in May and June 2022 were selected to analyze the clinical data of their clinical manifestations, laboratory examination, genetic test results, diagnosis and treatment and follow-up retrospectively. Results　Two children had typical clinical manifestations, such as café-au-lait spots, axillary freckles, intraocular iris hamartoma. Venous blood was collected from case 1 and his parents for NF gene test, and a new mutation of c.4084C>T in the NF1 gene was found, and their parents did not have the pathogenic gene; the venous blood of the children in case 2 was tested for whole-exome gene analysis, and a heterozygous nonsense variant c.910C>T:p.R304 on the NF1 gene was found, , which was verified by Sanger sequencing to be inherited from his mother, his mother has café-au-lait spots and brain glioma, and has undergone surgery to remove the brain glioma, but has not undergone chemoradiotherapy or targeted therapy. No neurological malignancies were detected in either of the two children at follow-up until July 2022. Conclusions　The clinical manifestations of NF1 are relatively typical, genetic testing is conducive to determine its classification, and regular follow-up review can help to detect and treat malignant tumors early, thus improving the patient's quality of life.

Secular trend in physical growth in Japanese children and adolescents during 1900-2016 / 中国学校卫生

Objective@#This paper analyses the secular trend in physical growth among Japanese children and adolescents from 1900 to 2016, and to provide scientific reference for growth and development in Chinese adolescents.@*Methods@#Data of height and weight of Japanese children and adolescents aged 6-17 years from 1900 to 2016 included in the Survey of School Health Care were used to illustrate growth rate and range of height, weight and BMI during different periods by using one-way ANOVA. Longitudinal correlation between height, weight and BMI was analyzed by using longitudinal analysis method.@*Results@#During 1900 to 2016, height growth ranged between 9.5-19.9 cm in boys and 10.8-18.9 cm in girls aged 6-17 years and the weight growth ranged between 4.0-15.9 kg in boys and 3.9-14.2 kg in girls, with males significantly higher than females; Peak height and weight growth rate of Japanese children and adolescents occurred between 1950 and 1960 (boys: 4.8 cm and 2.7 kg; girls: 3.9 cm and 2.4 kg). On the contrary, from 1939 to 1950, height and weight of Japanese children and adolescents showed a significant decreasing trend (boys: -1.8 cm and -1.2 kg per 10 years; girls: -0.8 cm and -0.4 kg per 10 years); During 2000-2016, the growth rates of height of boys and girls in all age groups in Japan were -0.2 to 0 cm in boys and -0.2 to -0.1 cm in girls per 10 years, respectively.@*Conclusion@#The long-term growth trend of Japanese children and adolescents shows an increasing trend before and after World War II; During World War II and the early post-war period, Japanese children and adolescents showed long-term decreasing trend. In the 21st century, the slow decreasing trend in growth among Japanese children and adolescents is observed, which might associate with absence of favorable environment.

Effect of lanthanum chloride on expressions of collagen protein in a wound tissue / 中华整形外科杂志

<p><b>OBJECTIVE</b>To evaluate the effect of lanthanum chloride on expressions of collagen protein and find a way to prevent and treat scar.</p><p><b>METHODS</b>Four linear incisions were made on the dorsal skin of an adult, female Sprague-Dawley rat as an animal model. One was non-manipulated as a control; the second was injected with distilled water as a sham-control; the third was injected with 50 mmol/L of lanthanum chloride, and the fourth was injected with 50 micrograms neutralizing antibody of TGF-beta 1 as a positive control. All of the wound tissues were harvested and assayed with ABC method in 14 days and 28 days after the surgery.</p><p><b>RESULTS</b>The expressions of type I, III and IV of collagen protein in the third group significantly reduced in 14 and 28 days after the operation, compared with the control or sham-control group. Its values wen as similar as the fourth group.</p><p><b>CONCLUSION</b>Lanthanum chloride could inhibit the expressions of collagen protein, and it may be used to prevent and treat scars.</p>

Effects of lanthanum chloride on the apoptosis of fibroblasts in trauma tissue / 中华烧伤杂志

<p><b>OBJECTIVE</b>To investigate the effects of lanthanum chloride on the apoptosis of fibroblasts in trauma tissue.</p><p><b>METHODS</b>Fifty adult female SD rats were used and linear incisions were made on the back near the joints of extremities of the rats. One of the cuts receiving no treatment was designated as blank control (C). 0.25 ml of distilled water, lanthanum chloride (50 mmol/L) and the antibody of (TGFbeta(1)) transforming growth factor beta(1) (0.2 mg/ml) were respectively injected into the both sides of the other three wounds subcutaneously and the wounds were divided into simulating control (SC), lanthanum chloride (LC) and antibody (A) groups. The fibroblast apoptosis in the wound tissue samples and the change in intracellular calcium concentration (Ca(2+)) were determined by flow cytometry (FCM) and TUNEL methods on the 14th and 28th day after the injection.</p><p><b>RESULTS</b>Apoptosis of fibroblasts was enhanced significantly after 14 days of injection in LC and A groups compared with that in C and SC groups (P < 0.05 approximately 0.01). Furthermore, intracellular Ca(2+) was increased evidently in LC group (P < 0.01).</p><p><b>CONCLUSION</b>It is indicated that lanthanum chloride might be effective in preventing scar development.</p>